牛胚胎干细胞研究进展

doi:10.11843/j.issn.0366-6964.2014.11.001

摘要/Abstract

摘要：

牛胚胎干细胞（Embryonic stem cells，ES）在畜牧业生产中具有重要的应用价值，可以用来研究早期胚胎的发育和培育新品种，对加速牛良种化进程具有重要理论意义和实践价值。国内外很多研究者为建立牛 ES 细胞系付出了巨大努力，并建立了一些牛类 ES 细胞系，但是迄今还未建立公认的牛 ES 细胞系。近年来，小鼠和人类干细胞体外培养及其功能研究取得了巨大进展，然而小鼠和人ES 细胞体外培养的条件并不适合培养牛 ES 细胞。影响牛 ES 细胞建系过程中的诸多因素如细胞因子、多能性标记基因与表面标记、传代方式和饲养层等还有待深入研究。本文综述了国内外牛 ES 细胞研究进展和影响牛 ES 细胞建系的主要因素，以期为牛 ES 细胞研究提供参考。

Abstract:

The study of bovine embryonic stem (ES) cells have shown that they have potential application in livestock industry.For instance，bovine ES cells could be used to study bovine early embryo development and would contribute to new varieties of bovine breed.Although it has been reported that many bovine embryonic stem cell-like cell lines have been got both in China and abroad，no validated bovine ES cell lines has been generated up to now.Researchers have achieved great progress in the study of mouse and human ES cells in recent years，and the culture conditions of the ES cells are relatively mature.But when used the same culture condition in bovine ES cells，the result is not so good.The generation of bovine ES cell lines could be affected by many factors，such as cytokines，pluripotent genes and surface markers，passage methods and feeder cells.This paper aims to summarize the current research progress of bovine ES cells at home and abroad，analyze some important factors affecting the generation of bovine ES cell lines and discuss its developing trends.

中图分类号:

S823.2

崔莉莎，赵学明，郝海生，杜卫华，朱化彬. 牛胚胎干细胞研究进展[J]. 畜牧兽医学报, 2014, 45(11): 1739-1745.

CUI Li-sha,ZHAO Xue-ming，HAO Hai-sheng，DU Wei-hua,ZHU Hua-bin . The Research Progress of Bovine Embryonic Stem Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1739-1745.

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